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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
16/12/2013 |
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Data da última atualização: |
16/12/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROLIM, A. A. S. G.; YANO, S. A. C.; SPECHT, A.; ANDRADE, C. G. T. de J.; SOSA-GOMEZ, D. R. |
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Afiliação: |
ADRIAN AUGUSTO SOSA GOMEZ ROLIM, ESALQ - USP; SILVIA AKIMI CAVAGUCHI YANO, CNPSo; ALEXANDRE SPECHT, CPAC; CELIA GUADALUPE TARDELLI DE JESUS ANDRADE, UEL; DANIEL RICARDO SOSA GOMEZ, CNPSO. |
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Título: |
Morphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Annals of the Entomological Society of America, v. 106, n. 5, p. 643-651, 2013. |
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DOI: |
10.1603/AN13049 |
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Idioma: |
Inglês |
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Conteúdo: |
The most important species of Lepidoptera that attack soybean, in descending order of importance, are Chrysodeixis includens (Walker), Anticarsia gemmatalis Hübner, Spodoptera cosmioides (Walker), Spodoptera eridania (Stoll), and Spodoptera albula (Walker). In addition, Rachiplusia nu (Guenée) is one of the most common Plusiinae in the southern region of Brazil, encompassing the states of Paraná, Santa Catarina, and Rio Grande do Sul. The correct identification of these species is essential for choosing the appropriate control measures, as they differ in their susceptibility to insecticides and biological control agents. To distinguish Lepidoptera species commonly found in Brazil, the eggshells of all these species were morphologically characterized by scanning electron microscopy. Diagnostic characters for differentiating species include shape of rosette petals, number of primary cells, number of secondary cells, number of ribs, and rosette diameter. Also, a molecular diagnostic method using polymerase chain reaction and restriction fragment length polymorphism (PCR?RFLP) analysis was developed to distinguish noctuid species commonly found in Brazil. A 658-bp region of the mitochondrial DNA cytochrome oxidase subunit I gene was amplified using PCR and then sequenced. The five Lepidoptera species were distinguished by restriction enzymes Bpm I and MboI. RFLPs produced by Bpm I endonuclease were useful to discriminate species from within Spodoptera (S. cosmioides, S. eridania, and S. albula) and R. nu from C. includens. However, Bpm I did not digest the amplicons from S. eridania and C. includens, which were discriminated by RFLP patterns produced by the restriction enzyme MboI. PCR-RFLP can be performed in a short period, and it is useful to distinguish the most important Brazilian Lepidoptera soybean pests. MenosThe most important species of Lepidoptera that attack soybean, in descending order of importance, are Chrysodeixis includens (Walker), Anticarsia gemmatalis Hübner, Spodoptera cosmioides (Walker), Spodoptera eridania (Stoll), and Spodoptera albula (Walker). In addition, Rachiplusia nu (Guenée) is one of the most common Plusiinae in the southern region of Brazil, encompassing the states of Paraná, Santa Catarina, and Rio Grande do Sul. The correct identification of these species is essential for choosing the appropriate control measures, as they differ in their susceptibility to insecticides and biological control agents. To distinguish Lepidoptera species commonly found in Brazil, the eggshells of all these species were morphologically characterized by scanning electron microscopy. Diagnostic characters for differentiating species include shape of rosette petals, number of primary cells, number of secondary cells, number of ribs, and rosette diameter. Also, a molecular diagnostic method using polymerase chain reaction and restriction fragment length polymorphism (PCR?RFLP) analysis was developed to distinguish noctuid species commonly found in Brazil. A 658-bp region of the mitochondrial DNA cytochrome oxidase subunit I gene was amplified using PCR and then sequenced. The five Lepidoptera species were distinguished by restriction enzymes Bpm I and MboI. RFLPs produced by Bpm I endonuclease were useful to discriminate species from within Spodoptera (S. cosmioides, S. eridania... Mostrar Tudo |
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Palavras-Chave: |
Lepidotero. |
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Thesagro: |
Phytophthora; Praga de planta; Pseudoplusia Includens; Soja; Spodoptera Eridania. |
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Thesaurus Nal: |
Brazil; Lepidoptera; Plant pests; Rachiplusia; Soybeans; Spodoptera albula; Spodoptera cosmioides. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94085/1/33766.pdf
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Marc: |
LEADER 02822naa a2200337 a 4500
001 1973974
005 2013-12-16
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1603/AN13049$2DOI
100 1 $aROLIM, A. A. S. G.
245 $aMorphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil.
260 $c2013
520 $aThe most important species of Lepidoptera that attack soybean, in descending order of importance, are Chrysodeixis includens (Walker), Anticarsia gemmatalis Hübner, Spodoptera cosmioides (Walker), Spodoptera eridania (Stoll), and Spodoptera albula (Walker). In addition, Rachiplusia nu (Guenée) is one of the most common Plusiinae in the southern region of Brazil, encompassing the states of Paraná, Santa Catarina, and Rio Grande do Sul. The correct identification of these species is essential for choosing the appropriate control measures, as they differ in their susceptibility to insecticides and biological control agents. To distinguish Lepidoptera species commonly found in Brazil, the eggshells of all these species were morphologically characterized by scanning electron microscopy. Diagnostic characters for differentiating species include shape of rosette petals, number of primary cells, number of secondary cells, number of ribs, and rosette diameter. Also, a molecular diagnostic method using polymerase chain reaction and restriction fragment length polymorphism (PCR?RFLP) analysis was developed to distinguish noctuid species commonly found in Brazil. A 658-bp region of the mitochondrial DNA cytochrome oxidase subunit I gene was amplified using PCR and then sequenced. The five Lepidoptera species were distinguished by restriction enzymes Bpm I and MboI. RFLPs produced by Bpm I endonuclease were useful to discriminate species from within Spodoptera (S. cosmioides, S. eridania, and S. albula) and R. nu from C. includens. However, Bpm I did not digest the amplicons from S. eridania and C. includens, which were discriminated by RFLP patterns produced by the restriction enzyme MboI. PCR-RFLP can be performed in a short period, and it is useful to distinguish the most important Brazilian Lepidoptera soybean pests.
650 $aBrazil
650 $aLepidoptera
650 $aPlant pests
650 $aRachiplusia
650 $aSoybeans
650 $aSpodoptera albula
650 $aSpodoptera cosmioides
650 $aPhytophthora
650 $aPraga de planta
650 $aPseudoplusia Includens
650 $aSoja
650 $aSpodoptera Eridania
653 $aLepidotero
700 1 $aYANO, S. A. C.
700 1 $aSPECHT, A.
700 1 $aANDRADE, C. G. T. de J.
700 1 $aSOSA-GOMEZ, D. R.
773 $tAnnals of the Entomological Society of America$gv. 106, n. 5, p. 643-651, 2013.
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Registro original: |
Embrapa Cerrados (CPAC) |
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| 1. |  | PIRES, J. L. F.; CORASSA, G. M.; RAMBO, A. C.; KERBER, L.; PASINATO, A.; DALMAGO, G. A.; SILVA JUNIOR, J. P. da; SANTI, A.; SANTI, A. L.; GUARIENTI, E. M.; CUNHA, G. R. da; STRIEDER, M. L.; FOCHESATTO, E. Aplicação de nitrogênio a taxa variável em cultura de trigo: estudo de caso na Unidade Piloto de Agricultura de Precisão de Não-Me-Toque, RS. In: BERNARDI, A. C. de C.; NAIME, J. de M.; RESENDE, A. V. de; BASSOI, L. H.; INAMASU, R. Y. (Ed.). Agricultura de precisão: resultados de um novo olhar. Brasília, DF: Embrapa, 2014. Cap. 26, p. 287-294.| Tipo: Capítulo em Livro Técnico-Científico |
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